eidd 2801 cat no t8309 powders  (TargetMol)

Journal: Viruses

Article Title: Molnupiravir and Its Active Form, EIDD-1931, Show Potent Antiviral Activity against Enterovirus Infections In Vitro and In Vivo

doi: 10.3390/v14061142

Figure Lengend Snippet: The molecular formula of EIDD−1931 and EIDD−2801 and the in vitro antiviral effects against EV−A71. ( A ) The molecular formula of EIDD−1931 and EIDD−2801. ( B – G ) The antiviral activities of EIDD−1931 ( B – D ) and EIDD2801 ( E – G ) against EV−A71 in different cells lines. RD cells, Vero cells, and Huh7 cells were infected with the EV−A71 H strain at 100 × TCID 50 . Different doses of the test compounds were then added. At 72 h.p.i, the antiviral parameters were measured. The antiviral effects and cytotoxicity of EIDD−1931 and EIDD−2801 were measured using a CellTiter−Glo cell viability assay kit. The EC 50 and CC 50 were calculated using Origin 9.0 software. SI = CC 50 /IC 50 .

Article Snippet: EIDD-1931(cat no. T8498) and EIDD-2801 (cat no. T8309) powders were purchased from TargetMol (Shanghai, China), and NITD008 (7-Deaza-2′-C-acetylene-adenosine, cat no. HY-12957) powders were purchased from MCE (Shanghai, China).

Techniques: In Vitro, Infection, Viability Assay, Software

Journal: Viruses

Article Title: Molnupiravir and Its Active Form, EIDD-1931, Show Potent Antiviral Activity against Enterovirus Infections In Vitro and In Vivo

doi: 10.3390/v14061142

Figure Lengend Snippet: Antiviral activities of EIDD−1931 and EIDD−2801 against EV−A71 in vitro. ( A – D ) EV−A71 virus particle yields and viral RNA reduction assay. RD cells were infected with EV71 at an MOI of 0.1 PFU/mL in the presence of different concentrations of EIDD−1931 or EIDD−2801. After 30 h, the virus particle yields in the supernatant were measured by TCID 50 . Total cellular RNA was extracted and subjected to qRT−PCR to measure viral RNA expression. All data are shown as means ± standard deviation from three independent experiments. Statistical significance was calculated using one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. ( E – G ) Inhibitory effects of EIDD-1931 and EIDD−2801 on viral VP1 protein. ( E ) RD cells were infected with EV−A71 at an MOI of 1 PFU/mL in the presence of EIDD−1931 or EIDD−2801. At 16 h.p.i., the cells were fixed for immunofluorescence assays. EV−A71 VP1 proteins were stained using mouse anti−EV−A71 antibody (12D7) (red), and cell nuclei were stained using Hoechst 33,342 (blue), scale bar: 50 µm. ( F , G ) RD cells infected with EV−A71 (MOI = 1) were treated with the test compounds at the indicated concentrations. At 24 h post infection, the cells were collected for Western blot analysis using anti−EV−A71VP1 antibodies (12D7). ( H , I ) Proteins levels of EV−A71 VP1 were quantified by ImageJ software. * p < 0.05, **** p < 0.0001.

Article Snippet: EIDD-1931(cat no. T8498) and EIDD-2801 (cat no. T8309) powders were purchased from TargetMol (Shanghai, China), and NITD008 (7-Deaza-2′-C-acetylene-adenosine, cat no. HY-12957) powders were purchased from MCE (Shanghai, China).

Techniques: In Vitro, Infection, Quantitative RT-PCR, RNA Expression, Standard Deviation, Immunofluorescence, Staining, Western Blot, Software

Journal: Viruses

Article Title: Molnupiravir and Its Active Form, EIDD-1931, Show Potent Antiviral Activity against Enterovirus Infections In Vitro and In Vivo

doi: 10.3390/v14061142

Figure Lengend Snippet: EIDD-1931 and EIDD-2801 exert in vivo antiviral effects against EV-A71 infection. Four groups of 1-day-old ICR suckling mice were i.p. infected with 10 6 PFU of EV-A71 virus (H strain), followed by i.p. treatment with EIDD-1931, EIDD-2801, or vehicle at the indicated dosages for 7 consecutive days. Survival ( A , C ) and body weight ( B , D ) were recorded every day until 21 d.p.i. Data for B and D are presented as means ± standard deviations. Survival data were analyzed with a log-rank test. ** p < 0.01, **** p < 0.0001.

Article Snippet: EIDD-1931(cat no. T8498) and EIDD-2801 (cat no. T8309) powders were purchased from TargetMol (Shanghai, China), and NITD008 (7-Deaza-2′-C-acetylene-adenosine, cat no. HY-12957) powders were purchased from MCE (Shanghai, China).

Techniques: In Vivo, Infection

Journal: Viruses

Article Title: Molnupiravir and Its Active Form, EIDD-1931, Show Potent Antiviral Activity against Enterovirus Infections In Vitro and In Vivo

doi: 10.3390/v14061142

Figure Lengend Snippet: EIDD-1931 and EIDD-2801 reduce the viral loads in various tissues of 1-day-old ICR suckling mice. Three groups of 1-day-old ICR suckling mice were i.p. infected with 10 6 PFU of EV-A71 virus (H strain), followed by i.p. treatment with EIDD-1931 and EIDD-2801 at a dosage of 200 mg/kg for 4 consecutive days. Then, the mice were euthanized, and brain ( A ), heart ( B ), intestine ( C ), liver ( D ), limb muscles ( E ) and lung ( F ) were separately harvested to determine the viral RNA loads using qRT-PCR. Data are presented as means ± standard deviations, and Student’s unpaired t test was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: EIDD-1931(cat no. T8498) and EIDD-2801 (cat no. T8309) powders were purchased from TargetMol (Shanghai, China), and NITD008 (7-Deaza-2′-C-acetylene-adenosine, cat no. HY-12957) powders were purchased from MCE (Shanghai, China).

Techniques: Infection, Quantitative RT-PCR